Assessment of molecular genetic diversity of ecuadorian rice cultivars using simple sequence repeat markers

Authors

  • Iris Pérez-Almeida Facultad de Ciencias Agrarias, Universidad de Guayaquil. Guayaquil EC090302 Ecuador
  • Roberto Celi-Herán Instituto Nacional de Investigaciones Agropecuarias, Estación Experimental Litoral Sur, Rice Program, Guayaquil EC092406 Ecuador
  • Fernando Sánchez-Mora Facultad de Ingeniería Agronómica, Campo Experimental La Teodomira, Universidad Técnica de Manabí. Lodana, EC 13132, Ecuador
  • Lenin Paz-Carrasco Instituto Nacional de Investigaciones Agropecuarias, Estación Experimental Litoral Sur, Rice Program, Guayaquil EC092406 Ecuador
  • Belén Ramos-Viteri Instituto Nacional de Investigaciones Agropecuarias, Estación Experimental Litoral Sur, Rice Program, Guayaquil EC092406 Ecuador

Keywords:

cluster análisis, molecular marker, Oryza sativa, rice breeding, variability

Abstract

Molecular markers are useful tools for evaluating genetic diversity and determining cultivar identity.  Thirty simple-sequence-repeat (SSR) markers were selected in order to evaluate the genetic diversity within 76 cultivars of the Ecuadorian Rice Program for breeding. One-hundred-ninety-four alleles were detected in 22 SSR polymorphic markers, number of alleles per marker ranging from 2 to 24, with an average of 9 alleles per locus.  The sizes of the alleles varied between 62 to 280 bp, with an average polymorphism information content value of 0.624, ranging from 0.202 (RM125) to 0.943 (RM413), indicating significant genetic diversity among and within the rice accessions. The average observed heterozygosity (Ho) was 0.086, while average expected genetic diversity (He) was 0.667. A set of eight of the polymorphic SSR markers produced seventeen unique alleles for the genotypes studied and could distinguish released cultivars from the rest of accessions.  A dendrogram constructed using the unweighted pair-group method with arithmetic means (UPGMA) grouped the 76 rice materials in four well differentiated major clusters whereas the Structure program without any a priori information provided support for the existence of three genetically distinct clusters (K = 3).

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Published

2020-03-28

Issue

Vol. 31 No. 1 (2019): Bioagro 31)(1)

Section

Artículos

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How to Cite

Assessment of molecular genetic diversity of ecuadorian rice cultivars using simple sequence repeat markers. (2020). Bioagro, 31(1), 3-12. https://revistas2.uclave.org/index.php/bioagro/article/view/2607